Mediators of Inflammation / 2019 / Article / Fig 3

Research Article

LincRNA Cox-2 Regulates Lipopolysaccharide-Induced Inflammatory Response of Human Peritoneal Mesothelial Cells via Modulating miR-21/NF-κB Axis

Figure 3

Suppression of Cox-2 ameliorating LPS induced HPMCs injury by regulation of miR-21 negatively. (a) Cox-2 negatively regulated the miR-21 expression after the HPMCs were transfected with pc-Cox-2, sh-Cox-2 and the corresponding controls. (b) The interference effect of miR-21 expression after the HPMCs were different transfected. (c) MTT assay showed cell viability after different treatments. (d) The expression of apoptosis-associated proteins after different transfections. (e) Cell apoptosis analyzed by Flow cytometry. (f) Flow cytometry showed cell apoptosis after different treatments. (g) The concentration of inflammatory factors in supernatant of HPMCs after different transfections. The experiments were repeated in triplicate. Significance: , and versus the controls.

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