Mediators of Inflammation / 2019 / Article / Fig 6

Research Article

Hydrogen Sulfide Attenuates High Glucose-Induced Human Retinal Pigment Epithelial Cell Inflammation by Inhibiting ROS Formation and NLRP3 Inflammasome Activation

Figure 6

H2S decreased high glucose-induced ROS production and inflammatory response in RPE cells. Intracellular ROS formation and inflammasome marker NLRP3, ASC, and caspase-1 expression were determined in ARPE-19 cells after 48 hours of culture under low and high glucose, with or without 200 μM of NaHS pretreatment. (a) The ROS production indicated by FACs in high-glucose culture; (b) the ROS production indicated by FACs in high-glucose+NaHS culture; (c) a bar graph of average results from the three individual experiments; (d) NLRP3 mRNA; (e) ASC mRNA; (f) caspase-1 mRNA. Data were presented as the error (SE) of three independent experiments. and , compared between the high-glucose and high-glucose+H2S groups (low glucose: 5.5 mM, high glucose: 25 mM).

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