Epigenetic Regulation of IL-17-Induced Chemokines in Lung Epithelial Cells

Figure 1

IL-17A induces proinflammatory chemokine production in primary human airway epithelial cells. (a) NHBE cells from 4 donors (derived from CORE donors) were established in the air-liquid interphase culture and treated with human recombinant IL-17A protein 100 ng/ml or control medium for 48 h. RT-PCR was performed to detect chemokine mRNA level. (b) Supernatant at 48 h was collected to measure the CXCL8 protein level by ELISA. (c) Heat map of the expression of multiple CXCR2 ligands and HDAC5 expression in normal HBE cell cultured in air-liquid interphase in the presence or absence of 100 ng/ml IL-17 in basal media for 48 h. ; ; ; by paired -test, one-tailed.

(a)

(b)

(c)