Epigenetic Regulation of IL-17-Induced Chemokines in Lung Epithelial Cells

Figure 4

Induction of proinflammatory chemokines mediated by IL-17A can be suppressed by overexpressing HDAC5. HBE1 cells were plated 0.08-0.15 million cells per well in a 12-well plate. When 70-80% confluent, cells were transfected with 0.1 moi human HDAC5 adenovirus and nontarget adenovirus in 300 μl medium, respectively, for one hour, and then we added 100 ng/ml h-IL-17A protein or 700 μl control medium directly into each corresponding well in both adenovirus-infected groups. 24 h after transfection, (a) HDAC5, (c) CXCL1, CXCL2, and CXCL8 mRNA levels were determined by RT-PCR. (d) 48 h supernatant after transfection was collected. CXCL8 protein level was achieved by ELISA. ; ; by paired -test, two-tailed. (b) HDAC5 protein level of cells with adenovirus transfection for 24 h was detected by Western blotting (β-actin as reference). This experiment has been repeated.

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