Butyrate Enhances Desensitization Induced by Oral Immunotherapy in Cow’s Milk Allergic Mice

Figure 4

Reduced activation of allergic effector cells upon challenge after OIT and butyrate or FOS supplementation. (a) Reduced acute allergic skin response (Δ ear swelling 1 h after i.d. injection with whey) in OIT + FOS and OIT + butyrate mice compared to sensitized controls. (b) Reduced mucosal mast cell degranulation (serum mMCP-1 concentration) in OIT + butyrate mice compared to sensitized controls. Decreased MFI of CD200R in basophils activated with (c) whey in OIT + butyrate blood samples compared to OIT and butyrate samples. No differences in MFI observed after basophil stimulation with (d) αIgE and (e) medium. Data are represented as mean ± SEM, /group (a, b) and /group (c, d) (whole blood samples were pooled per 2 mice). Statistical analysis was performed using one-way ANOVA and Bonferroni’s post hoc test to compare preselected combinations. , , , . i.d.: intradermal; i.g.: intragastric; mMCP-1: mucosal mast cell protease-1; MFI: median fluorescence intensity.

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