Mediators of Inflammation / 2019 / Article / Tab 1 / Research Article
Hoveniae Semen Seu Fructus Ethanol Extract Exhibits Anti-Inflammatory Activity via MAPK, AP-1, and STAT Signaling Pathways in LPS-Stimulated RAW 264.7 and Mouse Peritoneal Macrophages Table 1 Primary and secondary antibodies used for Western blot analysis.
Antibody Corporation Product no. Dilution rate iNOS Cell Signaling Technology #2977 1 : 1,000 COX-2 Cell Signaling Technology #4842 1 : 5,000 β -ActinSanta Cruz Biotechnology #SC-47778 1 : 5,000 P-ERK Cell Signaling Technology #4377 1 : 1,000 ERK Cell Signaling Technology #9102 1 : 1,000 P-p38 Cell Signaling Technology #9211 1 : 1,000 p38 Cell Signaling Technology #9212 1 : 1,000 P-JNK Cell Signaling Technology #9251 1 : 1,000 JNK Cell Signaling Technology #9252 1 : 1,000 P-c-Jun Cell Signaling Technology #9164 1 : 1,000 c-Jun Cell Signaling Technology #9165 1 : 1,000 TBP Cell Signaling Technology #8515 1 : 1,000 P-JAK2 Santa Cruz Biotechnology #SC-21870 1 : 500 JAK2 Santa Cruz Biotechnology #SC-294 1 : 500 P-STAT1 Cell Signaling Technology #7649 1 : 1,000 STAT1 Cell Signaling Technology #9172 1 : 1,000 P-STAT3 Cell Signaling Technology #9361 1 : 1,000 STAT3 Cell Signaling Technology #9139 1 : 1,000 NF-κ B p65 Cell Signaling Technology #3034 1 : 1,000 P-Iκ Bα Cell Signaling Technology #2859 1 : 1,000 Iκ Bα Cell Signaling Technology #4814 1 : 1,000 Secondary anti-mouse Cell Signaling Technology #7076 1 : 5,000 Secondary anti-rabbit Cell Signaling Technology #7074 1 : 5,000
