Hoveniae Semen Seu Fructus Ethanol Extract Exhibits Anti-Inflammatory Activity via MAPK, AP-1, and STAT Signaling Pathways in LPS-Stimulated RAW 264.7 and Mouse Peritoneal Macrophages

Table 2

Primers used for real-time RT-PCR.

Target gene	Primer sequence
TNF-α	F: 5-TTCTGTCTACTGAACTTCGGGGTGATCGGTCC-3
	R: 5-GTATGAGATAGCAAATCGGCTGACGGTGTGGG-3
IL-6	F: 5-TCCAGTTGCCTTCTTGGGAC-3
	R: 5-GTGTAATTAAGCCTCCGACTTG-3
IL-1β	F: 5-ATGGCAACTGTTCCTGAACTCAACT-3
	R: 5-CAGGACAGGTATAGATTCTTTCCTTT-3
iNOS	F: 5-GGCAGCCTGTGAGACCTTTG-3
	R: 5-GCATTGGAAGTGAAGCGTTTC-3
COX-2	F: 5-TGAGTACCGCAAACGCTTCTC-3
	R: 5-TGGACGAGGTTTTTCCACCAG-3
HO-1	F: 5-TGAAGGAGGCCACCAAGGAGG-3
	R: 5-AGAGGTCACCCAGGTAGCGGG-3
β-Actin	F: 5-AGAGGGAAATCGTGCGTGAC-3
	R: 5-CAATAGTGATGACCTGGCCGT-3

F: forward; R: reverse.