Neutrophils Are Dysregulated in Patients with Hereditary Angioedema Types I and II in a Symptom-Free Period

Figure 6

Decreases in CD25 expression/IFN-γ production on/in T-cell/PBMC of healthy donors (HD) and HAE patients after coculture with autologous neutrophils. (a, b) Coincubation of T-cells with autologous neutrophils in response to CD3/CD28 stimulation results in reduced percentages of CD25⁺ T-cells of total CD3⁺CD4⁺ or CD3⁺CD8⁺ T-cells in HAE patients. (c, d) Coincubation of T-cells with autologous neutrophils in response to the stimulation with CD3/CD28 results in reduced percentages of IFN-γ⁺ T-cells of total CD3⁺CD4⁺ or CD3⁺CD8⁺ T-cells in HAE patients. (e, f) PBMC coincubation with autologous neutrophils in response to stimulating with CD3/CD28 results in reduced CD25⁺ T-cell percentages of total CD3⁺CD4⁺ cells in HAE patients and CD3⁺CD8⁺ T-cell in HD. (g, h) Coincubation of PBMC with autologous neutrophils in response to stimulation with CD3/CD28 results in reduced percentages of IFN-γ on CD3⁺CD4⁺ or CD3⁺CD8⁺ T-cells in HAE patients and HD too (horizontal bars represent medians; whiskers: 10-90 percentile; stars represent the statistical significance: , , and .

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