Mediators of Inflammation / 2020 / Article / Fig 2

Research Article

IL-37 Exerts Anti-Inflammatory Effects in Fetal Membranes of Spontaneous Preterm Birth via the NF-κB and IL-6/STAT3 Signaling Pathway

Figure 2

IL-37 suppressed expression of inflammatory factors in human amniotic epithelial cells. (a, b) Dose-dependent effects of IL-37 (10 ng/ml, 50 ng/ml, and 100 ng/ml) on the mRNA and protein expression levels of IL-1β, IL-6, and TNF-α were measured by quantitative real-time PCR and ELISA. The detection range of the ELISA kit was 7.8-1000 pg/ml. The results were normalized to β-actin. (c, d) Time-dependent effects of 100 ng/ml IL-37 (0 h, 6 h, 12 h, 24 h) on the mRNA and protein expression levels of IL-1β, IL-6, and TNF-α were detected by quantitative real-time PCR and ELISA. The detection range of the ELISA kit was 7.8-1000 pg/ml. The results were normalized to β-actin. (e) Transfection efficiency of IL-37 siRNA was detected by quantitative real-time PCR and western blot. The results were normalized to β-actin. (f) The concentrations of IL-1β, IL-6, and TNF-α were measured by ELISA in the cell supernatant of the LPS-treated WISH cells after transfection with IL-37 siRNA for 48 hours and treatment with LPS and rhIL-37 for 24 hours, respectively. The detection range of the ELISA kit was 7.8-1000 pg/ml. ns: nonsignificance; vs. LPS; vs. LPS; vs. LPS; ; ## vs. control; ####.
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