The Effects of Targeted Temperature Management on Oxygen-Glucose Deprivation/Reperfusion-Induced Injury and DAMP Release in Murine Primary Cardiomyocytes
Increasing duration of OGD exposure resulted in a transition from apoptotic to necrotic cell death in the murine primary cardiomyocytes that could be attenuated by TTM. (a) OGD-induced toxicity as measured by LDH release at 2, 4, and 6 hours is presented as % cytotoxicity relative to positive (100%) and normoxia (0%) controls. (b) Apoptotic programmed cell death was assessed by cleavage of caspase-3 after exposure to 2, 4, or 6 hours OGD followed by 6 hours of reperfusion and is presented as -fold change relative to normoxia control at the respective experimental time points. Data from at least 3 independent experiments are shown as , and statistical analysis was performed using one-way ANOVA with Tukey posttest; # as compared to 37°C normoxia control group and for comparisons between the two experimental groups were considered significant.
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