CD4⁺ T Cell Profile and Activation Response in Sickle Cell Disease Patients with Osteonecrosis

Figure 2

Relative frequency of major T cell populations in bone marrow mononuclear cells (BM-MNC) from SCD patients with osteonecrosis (SCD BM) and patients with osteonecrosis not related to SCD (non-SCD BM). Representative dot plots illustrate the strategy of gating for major T cell subsets defined by CD3⁺CD4⁺ (a), CD3⁺CD8⁺ (b), CD4⁺/45RA⁺ (c), and TCD4⁺/45RO⁺ (d) surface expressions in BM-MNC. All gates were based on isotype-matched control staining. TCD4⁺ and TCD8⁺ cells are lower in SCD BM. Cumulative data showing the frequencies of total TCD4⁺ (e), total TCD8⁺ (f), TCD4⁺CD45RA⁺ (g), and TCD4⁺CD45RO⁺ (h) subpopulations within live-gated single cells. Each symbol represents an individual subject. Horizontal lines represent mean and standard error. ; .

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