Mediators of Inflammation / 2020 / Article / Fig 2

Research Article

CD4+ T Cell Profile and Activation Response in Sickle Cell Disease Patients with Osteonecrosis

Figure 2

Relative frequency of major T cell populations in bone marrow mononuclear cells (BM-MNC) from SCD patients with osteonecrosis (SCD BM) and patients with osteonecrosis not related to SCD (non-SCD BM). Representative dot plots illustrate the strategy of gating for major T cell subsets defined by CD3+CD4+ (a), CD3+CD8+ (b), CD4+/45RA+ (c), and TCD4+/45RO+ (d) surface expressions in BM-MNC. All gates were based on isotype-matched control staining. TCD4+ and TCD8+ cells are lower in SCD BM. Cumulative data showing the frequencies of total TCD4+ (e), total TCD8+ (f), TCD4+CD45RA+ (g), and TCD4+CD45RO+ (h) subpopulations within live-gated single cells. Each symbol represents an individual subject. Horizontal lines represent mean and standard error. ; .

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