Mediators of Inflammation / 2020 / Article / Fig 3

Research Article

CD4+ T Cell Profile and Activation Response in Sickle Cell Disease Patients with Osteonecrosis

Figure 3

Increased frequency of IL4+CD4+ T cells and bifunctional IFN-γ+/ IL4+ and IL-17+/ IL4+CD4+ T cells in SCD and SCD/ON compared to healthy controls. Peripheral blood mononuclear cells from healthy donors (control), steady-state SCD, and SCD with osteonecrosis (SCD/ON) patients were culture stimulated with PMA/Io and stained for intracellular cytokine production. Representative dot plots illustrate intracellular staining for IFN-γ+/IL-4+ (a) and IL-4+/IL-17+ (b) in TCD4+ cells after stimulation. Relative frequency of IFN-γ+ (c), IL-4+ (d), IL-17+ (e), bifunctional IFN-γ+/IL-4+ (f), and IL-4+/IL-17+ TCD4+ (g) cells producing cytokines in relation to total TCD4+ cells in culture. Each symbol represents an individual subject. Horizontal lines represent mean and standard error. , , and .

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