Protection of Fecal Microbiota Transplantation in a Mouse Model of Multiple Sclerosis

Figure 4

FMT confers protection on myelin and axons in EAE. (a) MBP and NF-L protein expression in the brain of EAE controls and FMT-treated EAE mice shown by Western blot with β-tubulin as loading control. (b, c) Charts of quantified Western blot results showing increased levels of MBP (b) and decreased levels of NF-L (c) expression (normalized by β-tubulin) after FMT treatment (; /group; and ). (d) Immunofluorescence imaging of MBP (green) and NF-L (red) expression in the corpus callosum of the mouse brain. Nuclear staining was by Hoechst (blue). Each rightmost panel shows a high magnification image of the area inside the white box. Scale bar: 25 μm. (e) Transmission electron microscopy (TEM) imaging of myelin sheaths in the thoracic spinal cord. Each high magnification image (right) shows the area enclosed by the white box (left). Representative normal and damaged myelin sheaths are indicated by arrowheads and arrows, respectively.

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