Pirfenidone Inhibits Hypoxic Pulmonary Hypertension through the NADPH/ROS/p38 Pathway in Adventitial Fibroblasts in the Pulmonary Artery

Figure 3

PFD significantly inhibited the mRNA and protein expression of a-SMA and procollagen I (COL1A1). (a, b) The mRNA expression of a-SMA and procollagen I (COL1A1) was assessed by RT-PCR. (c–e) The protein expression of a-SMA and procollagen I (COL1A1) was assessed by western blot analysis. Nor: normoxia; Hyp: hypoxia; PFD: pirfenidone; a-SMA: smooth muscle a-actin.

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