TLR4/AP-1-Targeted Anti-Inflammatory Intervention Attenuates Insulin Sensitivity and Liver Steatosis

Figure 2

TLR4/AP-1 siRNA transfection ameliorated high-fat diet-induced obesity and insulin resistance in mice. No significant difference was observed in energy intake between NC, HM, HC, HT, and HA (a). Body weight, the weight of epididymal fat (eWAT), and wet weight of the liver were significantly increased in HM and HC compared with NC, and these changes were reversed in HT and HA (b–d). The glucose tolerance (GTT) and systemic (fasting plasma insulin level and ITT) and hepatic (Akt phosphorylation in the liver) insulin sensitivity were significantly impaired in HM and HC, and these changes were relieved in HT and HA (e–j). and compared with the NC group; # and ## compared with the HM group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)