CD19⁺CD24^hiCD38^hi B Cell Dysfunction in Primary Biliary Cholangitis

Figure 4

CD19⁺CD24^hiCD38^hi B cells from PBC patients express lower level of IL-10 when stimulated by anti-BCR and CpG, compared to HC subjects. PBMC from PBC and healthy individuals were stimulated for 48 h with or without anti-BCR and CpG. Phorbol ester, plus ionomycin, plus Brefeldin A were added for the last 5 h of culture. Cells were surface stained with CD19, CD24, and CD38 monoclonal antibodies (mAbs) and intracellularly stained for IL-10. (a, c) Representative intracellular IL-10 staining in unstimulated or stimulated CD19⁺CD24^hiCD38^hi B cells in the peripheral blood of one PBC patient and healthy control. (b, d) Statistical graph of the frequency of IL-10⁺ cells in unstimulated or stimulated CD19⁺CD24^hiCD38^hi B cells from PBC patients and HC subjects; .

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