Anti-Inflammatory and Antioxidant Effects of Carpesium cernuum L. Methanolic Extract in LPS-Stimulated RAW 264.7 Macrophages
CLME prevents septic death in an LPS-induced endotoxemia mouse model and inhibits the levels of NO, PGE2, and iNOS in LPS-stimulated RAW 264.7 cells. (a) The graph represents survival rate after LPS injection (25 mg/kg) with or without CLME treatment (50 or 100 mg/kg). RAW 264.7 cells were pretreated with indicated concentrations of CLME for 1 h and followed by LPS stimulation (1 μg/mL) for indicated times. (b) NO production was analyzed indirectly by measuring the supernatants of cultured RAW 264.7 cells for nitrite using the Griess reagent. (c) The prostaglandin E2 (PGE2) production in the culture media was measured by ELISA kit. (d) The iNOS expression was analyzed by western blot analysis. Densitometric analysis was performed using ImageJ ver. 1.50i. The values are represented as the ().### vs. the CON group; vs. the LPS-treated group.
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