Research Article

Anti-Inflammatory and Antioxidant Effects of Carpesium cernuum L. Methanolic Extract in LPS-Stimulated RAW 264.7 Macrophages

Figure 3

CLME inhibits the generation of ROS in LPS-stimulated RAW 264.7 cells. RAW 264.7 cells were pretreated with indicated concentrations of CLME for 1 h and followed by LPS stimulation (1 μg/mL) for 1 h. (a) The plot shows the histogram of gated cells with two markers providing data on two cell populations: ROS neg. (-) and ROS pos. (+) cells, indicating M1 and M2, respectively. ROS generations were analyzed by MUSE® oxidative stress assay kit. (b) The graph for percentage of ROS pos. (+) cells. The values are represented as the (). ## vs. the CON group; vs. the LPS-treated group.
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