Anti-Inflammatory and Antioxidant Effects of Carpesium cernuum L. Methanolic Extract in LPS-Stimulated RAW 264.7 Macrophages

Figure 4

CLME regulates the expression of HO-1 and Keap1/Nrf2 in LPS-stimulated RAW 264.7 cells. (a–c) HO-1 expression in RAW 264.7 cells. (a) The cells were treated with CLME of 25 μg/mL for the indicated times. (b) The cells were treated with CLME of indicated concentrations for 12 h. The values are represented as the (). and vs. the CON group. (c) The cells were pretreated with indicated concentrations of CLME for 1 h and followed by LPS stimulation (1 μg/mL) for 12 h. (d, e) The cells were pretreated with indicated concentrations of CLME for 1 h and followed by LPS stimulation for 1 h. (d) Keap1 and (e) Nrf2 expressions were analyzed by western blot analysis. Densitometric analysis was performed using ImageJ ver. 1.50i. The values are represented as the (). ^### vs. the CON group; and vs. the LPS-treated group.

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