Intravenous Arginine Administration Downregulates NLRP3 Inflammasome Activity and Attenuates Acute Kidney Injury in Mice with Polymicrobial Sepsis

Figure 4

Protein levels of the nucleotide-binding domain-like receptor protein 3 (NLRP3) inflammasome complex in renal tissues. (a) Protein expressions of NLRP3, apoptosis-associated speck-like protein containing CARD (ASC), interleukin (IL)-1β, and caspase-1. Whole-tissue lysates were analyzed by immunoblotting, and β-actin was used as a loading control. (b) Densitometric analysis of blots corrected by the protein loading control. NC: normal control group; Sham: sham group; SS: sepsis group with saline injection sacrificed at 6, 12, and 24 h after cecal ligation and puncture (CLP); SA: sepsis group with arginine injection sacrificed at 6, 12, and 24 h after CLP. Results of the densitometric analysis are presented as the ; for each group. Differences among groups were analyzed using a one-way ANOVA with Tukey’s post hoc test. Significantly differs from the NC group; ^#significantly differs from SS groups at the same time point ().

(a)

(b)