Cryptococcus neoformans Secretes Small Molecules That Inhibit IL-1β Inflammasome-Dependent Secretion

Figure 1

Exopolysaccharide incorporation on an acapsular mutant does not impair macrophage inflammasome activation. (a) GXM detection by immunofluorescence of B3501, CAP67 (Δcap67), and CAP67+PS (Δcap67 coated with polysaccharides from B3501) labeled with Uvitex2B (blue) and mAb against GXM (18B7) (red). (b) BMDMs were stimulated with LPS (500 ng/ml) and infected with opsonized B3501, Δcap67, or Δcap67+PS strains (MOI 2 : 1) for 24 h. Statistical analysis was performed using one-way ANOVA, where . Comparisons were made with the B3501-infected group.

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