Cryptococcus neoformans Secretes Small Molecules That Inhibit IL-1β Inflammasome-Dependent Secretion

Figure 7

ILA and HPLA increased fungal survival in Acanthamoeba castellanii and mice experimentally infected. Intracellular growth (CFU of 3 h vs. 24 h postinfection) inside A. castellanii was measured by CFU after treatment with 1 kDa CM35, MM, and ILA associated or not with PLA and HPLA. (b) The survival curve of C57Bl/6 infected with H99 strain treated with 1 kDa CM35 or MM during each 5 days intranasally. (c) The lung fungal burden from C57Bl/e infected with H99 strain treated with 1 kDa CM35, MM, or ILA+HPLA each 5 days intranasal repeated after 15 days postinfection. (d) IL-1β release in lung tissue after 15 days postinfection from C57Bl/e infected with H99 strain treated with 1 kDa CM35, MM, or ILA+HPLA each 5 days intranasal repeated. 1 kDa CM35 = conditioned media from C. neoformans B3501; MM = minimal media; PLA = DL-3-Phenyllactic acid; HPLA = DL-p-Hydroxyphenyllactic acid; ILA = DL-Indole-3-lactic acid. Statistical analysis was made utilizing one-way ANOVA, where ns: not significant; ; ; . Comparisons were made with the PBS group (a, b). Comparisons were made with the (c) MM or (d) PBS group.

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