The Depletion of ABI3BP by MicroRNA-183 Promotes the Development of Esophageal Carcinoma

Figure 4

ABI3BP may be one of the targets of miR-183 in Eca-109 and KYSE-150 cells. (a) Predicted consequential pairing site of ABI3BP mRNA and miR-183-3p. (b) Dual-luciferase reporter assay was performed to detect the luciferase activity of ABI3BP mRNA and miR-183-3p. Three independent experiments () were repeated. (c) Two kinds of cells were cultured and harvested when growing to 80-90%. Total protein was extracted and detected by Western blot analysis. Relative ABI3BP protein level was detected by Western blot under miR-183-3p overexpression condition. Five independent experiments () were repeated for each cell line. (d) Quantitative analysis of (c). , Student’s -test.

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