Reevaluation of Lung Injury in TNF-Induced Shock: The Role of the Acid Sphingomyelinase

<div>Kidney injury. Representative PAS-stained kidney sections from (a–c) WT mice and (d–f) ASM<sup>-/-</sup> mice treated as indicated above; scale bars 50 <i>μ</i>m, magnification 400x. (g) The total tubular injury score. The tubular injury was scored in (h) WT and (i) ASM<sup>-/-</sup> mice on a scale of 0–4: 0 = normal histology, 1 = mild dilatation, 2 = flattened epithelial cells and loss of brush border, 3 = mild vacuolization of tubular cytoplasm (&lt;5 vacuoles/tubule) and tubular cell necrosis/apoptosis; 4 = strong vacuolization of tubular cytoplasm (&gt;5 vacuoles/tubule) and tubular cell necrosis/apoptosis. (j) The tubular injury marker KIM-1 was analyzed in urine samples by western blot. 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Mediators of Inflammation

fig6

Figure 6

Figure 6: Reevaluation of Lung Injury in TNF-Induced Shock: The Role of the Acid Sphingomyelinase 