Micheliolide Attenuates Lipopolysaccharide-Induced Inflammation by Modulating the mROS/NF-κB/NLRP3 Axis in Renal Tubular Epithelial Cells

Figure 3

MCL inhibits LPS+ATP-induced activation of the NLRP3 inflammasome in NRK-52E cells. (a, b) Western blot analysis of the NLRP3 expression and its relative expression levels normalized to β-actin (ACTB). (c, d) Western blot analysis of the caspase-1 p10 expression and its relative expression levels normalized to α-tubulin. (e, f) Western blot analysis of the IL-1β expression and its relative expression levels normalized to α-tubulin. (g) Real-time PCR analysis of IL-1β expression in renal tubular epithelial cells. (h, i) Western blot analysis of the IL-18 expression and its relative expression levels normalized to ACTB. (j) ELISA analysis of IL-18 expression in each group. Data are presented as the . , versus normal controls; , ^## versus the LPS+ATP stimulation group.

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