IL-33 Mediates Lung Inflammation by the IL-6-Type Cytokine Oncostatin M

Figure 1

AdOSM-induced upregulation of arginase-1 (Arg1) protein expression in the mouse lung is IL-33-dependent. C57Bl/6 mice were endotracheally administered AdDl70 (control) or AdOSM and whole lung cell lysates analyzed for the expression of IL-33, phospho-STAT3/total STAT3 (pSTAT3/tSTAT3), and arginase-1 (Arg1) after 7 and 14 days by Western blotting. (a) IL-33, Arg1, phospho-STAT3 (pSTAT3), and beta-actin (Actin) expression in C57Bl/6 wild-type mice. (b, c) IL-33, pSTAT3/tSTAT3, Arg1, and beta-actin (Actin) expression in C57Bl/6 wild-type or IL-33-/- (IL-33-/-) mice at day 7. (d–f) Densitometry analysis of IL-33 and Arg1 signal relative to beta-actin levels or pSTAT3 relative to total STAT3 (tSTAT3), in C57Bl/6 mouse lung data shown in (a) or (b/c), respectively. AU: arbitrary units; : 3 mice/group in (a) and 5-7 mice/group in (b/c). .

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