IL-33 Mediates Lung Inflammation by the IL-6-Type Cytokine Oncostatin M

Figure 2

AdOSM-induced upregulation of mouse lung IL-33 mRNA and localization of IL-33 and OSM-mRNA expressing lung cells. C57Bl/6 wild-type (WT) or IL-33-/- mice were endotracheally administered AdDl70 (control) or AdOSM and analyzed after 7 days for IL-33 and TSLP mRNA expression from whole lung by qPCR (a). IL-33 and OSM mRNA expression was assessed within histological lung sections by chromatographic in situ hybridization (CISH) in AdDl70 (b), AdOSM in WT (c, right panel showing higher magnification) or AdOSM in IL-33-/- mice (d, right panel showing higher magnification). Red arrowheads: OSM+ mRNA signal; green arrowheads: IL-33+ mRNA signals.

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