IL-33 Mediates Lung Inflammation by the IL-6-Type Cytokine Oncostatin M

Figure 8

IL-33 stimulates production of IL-6-type cytokines (OSM, IL-6, and LIF) and arginase-1 activity from macrophages. (a) RAW264.7 cells were stimulated with varying doses of recombinant mouse IL-33 (0-50 ng/ml). After 48 hours, cell culture media was collected and assayed for the presence of OSM, IL-6, LIF, and TNFalpha (TNFa) by ELISA. Four replicates/treatment. (b) Bone marrow-derived macrophages (BMDM) were differentiated into M1- (LPS+IFNγ) or M2-type (IL-4+IL-13) macrophages or remained untreated (M0) and stimulated with 5 or 50 ng/ml of IL-33 for 24 hours. Cell lysates were assayed for arginase-1 activity (urea production) and supernatants assayed by ELISA for Oncostatin M (OSM). (c) BMDM cells from wild-type or IL-33-/- mice were differentiated into M1 or M2 cells or treated with LPS alone with or without IL-6 (a potentiator of M2 cells) and assayed for arginase-1 activity or nitric oxide production. Four replicates/treatment. , .

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