Regulation of Cyclooxygenase 2 by Filifactor alocis in Fibroblastic and Monocytic Cells

Figure 3

(a) Expression of COX2 in the presence and absence of F. alocis (OD₆₆₀: 0.1) in THP-1 cells at 1 d and 2 d, as analyzed by qPCR. (). Significant () difference between groups. (b) Stimulation of COX2 expression by various concentrations of F. alocis (OD₆₆₀: 0.05, 0.1, and 0.2) in THP-1 cells at 1 d, as analyzed by qPCR. Unstimulated cells served as the control. (). Significantly () different from the control. (c) COX2 protein level in lysates of THP-1 cells in the presence and absence of F. alocis (OD₆₆₀: 0.1) at 1 d, as analyzed by ELISA. (). Significant () difference between groups. (d) Expression of COX2 in the presence and absence of TNFα (1 ng/ml) in THP-1 cells at 1 d and 2 d, as analyzed by qPCR. (). Significant () difference between groups.

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