Monocytes Undergo Functional Reprogramming to Generate Immunosuppression through HIF-1α Signaling Pathway in the Late Phase of Sepsis

Figure 3

Its expression of proteins in the monocytes. (a) The protein bands of HIF-1α, STAT3, and PD-L1 in the clinical study. (b) The downregulated expression of proteins HIF-1α and STAT3 in the late phase of sepsis compared with the primary phase of sepsis; ^# represented significant difference. While the upregulation of the immune checkpoint protein PD-L1 expressed on the monocytes in the late phase of sepsis, ^# represented significant difference vs. the primary phase of sepsis. of proteins HIF-1α and STAT3 in the primary phase of sepsis versus the normal control group. (c) The protein bands of HIF-1α, STAT3, and PD-L1 in the sepsis rat model study. (d) Upregulated expression of proteins HIF-1α and STAT3 and downregulated protein PD-L1 expression in the sepsis rat model with Ade-HIF-1α injection; besides the highly expressed protein PD-L1 in the sepsis rats model, ^## of PD-L1 and HIF-1α versus the normal control group and ov-HIF-1α adenovirus-injected group, of PD-L1 versus the normal control group; of HIF-1α and STAT3 versus the normal control group, sepsis rat model group, and adenovirus control group; ^# of STAT3 versus the normal control group and ov-HIF-1α adenovirus-injected group; data were presented as , and analysis was replicated in triplicate.

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