IκB Kinase Inhibitor VII Modulates Sepsis-Induced Excessive Inflammation and Cardiac Dysfunction in 5/6 Nephrectomized Mice

Figure 3

Treatment of LPS (2 mg/kg) elevates the phosphorylation of IKKα/β and IkBα, the nucleus translocation of NF-κB, and levels of iNOS. 5/6 nephrectomized sham mice or 5/6 nephrectomized mice were given either LPS (2 mg/kg) or vehicle (PBS) via intraperitoneal route. The signaling effects on cardiac tissue were evaluated after 18 hours. The optical density was assessed. (a) Optical density of phosphorylated IKKα/β against total IKKα/β levels normalized against corresponding sham band. (b) Optical density of phosphorylated IkBα against total IkBα levels normalized against corresponding sham band. (c) Levels of NF-κB in nuclear and cytosolic fractions presented as nucleus/cytosol ratio; the results were normalized against corresponding sham band. (d) Levels of iNOS normalized against actin as loading control. All the analysis was performed in triplicate. compared to 5/6 nephrectomized sham mice with described treatment; ^# compared to the corresponding PBS group.

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