IκB Kinase Inhibitor VII Modulates Sepsis-Induced Excessive Inflammation and Cardiac Dysfunction in 5/6 Nephrectomized Mice

Figure 4

CLP enhances the phosphorylation levels of IKKα/β and IkBα, the nucleus translocation of NF-κB, and expression of iNOS. 5/6 nephrectomized sham or 5/6 nephrectomized mice were submitted to CLP or sham surgery. The signaling effects on cardiac tissue were evaluated after 24 hours. The optical density was assessed. (a) Optical density of phosphorylated IKKα/β against total IKKα/β levels normalized against corresponding sham band. (b) Optical density of phosphorylated IkBα against total IkBα levels normalized against corresponding sham band. (c) Levels of NF-κB in nuclear and cytosolic fractions presented as nucleus/cytosol ratio; the results were normalized against corresponding sham band. (d) Levels of iNOS normalized against actin as loading control. All the analysis was performed in triplicate. compared to 5/6 nephrectomized sham mice with described treatment; ^# compared to the corresponding sham-operated group.

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