CD137 Signaling Promotes Endothelial Apoptosis by Inhibiting Nrf2 Pathway, and Upregulating NF-κB Pathway

Figure 4

CD137 signaling promotes intracellular ROS generation and proinflammatory cytokines production by inhibiting Nrf2 pathway. (a) Analysis of ROS production by HUVECs using the DCFH-DA assay. Photographs were taken at ×100 magnification. (b) Summarized data. (c) Expression of IL-6, IL-1β, and TNF-α in HUVECs, as determined by RT-qPCR. (d) Expression of IL-6, IL-1β, and TNF-α in the supernatant of HUVECs, as determined by ELISA. Values are presented as the from three independent experiments. , , vs. control; ^#, ^##, ^### vs. agonist-CD137 group; ^& vs. M5580 group.

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