CD137 Signaling Promotes Endothelial Apoptosis by Inhibiting Nrf2 Pathway, and Upregulating NF-κB Pathway

Figure 5

CD137 signaling promotes the translocation and phosphorylation of NF-κB. (a) Images demonstrate a change in the color of the nucleus from red to yellow (due to colocalization of green FITC fluorescence and red propidium iodide fluorescence) which is indicative of NF-κB translocation in HUVECs. Scale bars represent 100 μm in ×200 magnifications. (b) Summarized data. (c) Western blot analysis tested the NF-κB nuclear and cytosolic expression. (d) Western blot analysis tested the expression of p-NF-κB. Values are presented as the from three independent experiments. , , vs. control; ^#, ^##, ^### vs. agonist-CD137 group.

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