miR-10a in Peripheral Blood Mononuclear Cells Is a Biomarker for Sepsis and Has Anti-Inflammatory Function

Figure 7

Effect of miR-10a overexpression on LPS-induced inflammatory cytokine production in monocytes. THP-1 monocytes were transduced with lentiviral miR-10a or miR-control. (a) Four days after the transduction, miR-10a levels in THP-1 cells were determined via qRT-PCR. (b) MAP3K7 expression in transduced THP-1 cells was determined via Western blot analysis. (c) The transduced cells were treated with or without LPS (100 ng/ml) for 24 h. Levels of IL-6, TNF-α, and MCP-1 in treated cells were assayed via qRT-PCR. Data are presented as , . .

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(b)

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