Systemic Administration of Calea pinnatifida Inhibits Inflammation Induced by Carrageenan in a Murine Model of Pulmonary Neutrophilia

Table 1

Effects of CE, fractions, and isolated compounds obtained from C. pinnatifida leaves upon relative fold change of leukocytes and exudate protein concentration in a murine model of carrageenan-induced pleurisy.

Groups (mg/kg)	Relative fold change of leukocytes	Relative fold change of neutrophils	Exudate protein concentration (μg/mL)
Sal^a	—	—
Cg^a
CE (25)^b
CE (50)^b
CE (100)^b
Hex (5)^b
Hex (10)^b
Hex (25)^b
MeOH (5)^b
MeOH (10)^b
MeOH (25)^b
EtOAc (2.5)^b
EtOAc (5)^b
EtOAc (10)^b
EtOAc (25)^b
3,5-diCQA (1)^b
3,5-diCQA (2.5)^b
3,5-diCQA (5)^b
4,5-diCQA (1)^b
4,5-diCQA (2.5)^b
4,5-diCQA (5)^b
Dex (0.5)^b
Indo (5)^b

Crude extract (CE: 25–100 mg/kg), hexane fraction (Hex: 5–25 mg/kg), methanol fraction (MeOH: 5–25 mg/kg), ethyl acetate fraction (EtOAc: 2.5–25 mg/kg), 3,5-di-O-E-caffeoylquinic acid (3,5-diCQA: 1–5 mg/kg), and 4,5-di-O-E-caffeoylquinic acid (4,5-diCQA: 1–5 mg/kg) obtained from C. pinnatifida leaves administered 0.5 h before pleurisy induction. Sal: negative control group, animals treated only with sterile saline (0.95%); Cg: positive control group, animals treated only with carrageenan (1%); Dex: animals treated with dexamethasone (0.5 mg/kg) 0.5 h before pleurisy induction; Indo: animals treated with indomethacin (5 mg/kg) 0.5 h before pleurisy induction. ^aAdministered by intrapleural route (i.pl.). ^bAdministered by intraperitoneal route (i.p.). Each group represents the of six animals. and .