Global Transcriptional Profiling Reveals Novel Autocrine Functions of Interleukin 6 in Human Vascular Endothelial Cells

Figure 5

Specific blockage of IL6-transsignaling does not affect genes differentially regulated after IL6 knockdown in human endothelial cells. Soluble gp130 Fc fusion protein (sgp130Fc) was used to evaluate whether specific blockage of IL6 transsignaling affects the gene expression of 3 selected genes differentially regulated after IL6 knockdown. sgp130Fc does not affect the phosphorylation of STAT3 induced by IL6 classic signaling (a, c) but inhibits phosphorylation of STAT3 induced by IL6 transsignaling (IL6+sIL6R) (b, d). Gene expression of three genes differentially regulated after IL6 knockdown (NEK7, TEMEM87B, and ESCO2) was not affected by sgp130Fc (e). Data from 3 to 4 independent experiments are shown. Phosphorylation of STAT3 was evaluated after 30 min treatments and gene expression after 48 h. IL6 was used at a concentration of 50 ng/ml, sIL6R at 10 ng/ml, and sgp130Fc at 1 μg/ml. , , and .

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