Global Transcriptional Profiling Reveals Novel Autocrine Functions of Interleukin 6 in Human Vascular Endothelial Cells

Figure 8

Merged network showing the interplay between IL6-mediated signaling and interaction, binding, and adhesion of endothelial cells. Using Ingenuity Pathway Analysis (IPA), a merged network was generated by connecting genes known to be central for IL6-mediated signaling (IL6, IL6R, IL6ST, STAT3, JAK1, JAK2, and SOCS3) with 42 differentially regulated genes extracted from the enriched functions related to binding, adhesion, and interaction of endothelial cells which were predicted to be activated by IL6 knockdown. Downregulated genes are indicated in green while upregulated genes are indicated in red using a fold change of 1.5 and Benjamini-Hochberg’s estimated . Grey symbols indicate expressed genes which were not differentially regulated after IL6 knockdown, while white symbols indicate nonexpressed genes. Purple lines indicate the interaction between genes known to be central for IL6 signaling.