Molecularly Distinct NLRP3 Inducers Mediate Diverse Ratios of Interleukin-1β and Interleukin-18 from Human Monocytes

Figure 3

Caspase-1 activity and cell lysis do not correlate with the IL-1β : IL-18 ratio. Caspase-1 activity was measured by flow cytometry using the FLICA probe (a), while LDH assay was used as a measure of cell rupture (b). Pro-IL-1β, cleaved IL-1β, IL-18, and caspase-1 p20 were detected in the extracellular fraction (EF) or cellular lysate as the cellular fraction (CF) by Western blot. The blot density was normalized against the total protein load to calculate the fraction (c). Representative blots are shown in (d). Measurement took place 24 h after stimulation, and controls were treated with LPS alone. Data are shown as from six individual experiments. ; .

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