Interleukin-22 Polymorphisms in Plasmodium falciparum-Infected Malaria Patients

Figure 1

Luciferase activity assay for fragments containing the promoter of IL-22 gene. (a) Nested deletions of the promoter region were generated and cloned. The clones were then transfected in HEK293 cell line for 48 h. Cells were harvested and tested for luciferase activity. (b) Two constructs of fragment F3R1 containing SNP rs2227513 were generated. One clone harbored A allele and the other clone contained G allele of SNP rs2227513. Luciferase activity was performed as shown in (a).

(a)

(b)