Nicotine Synergizes with High-Fat Diet to Induce an Anti-Inflammatory Microenvironment to Promote Breast Tumor Growth

Figure 2

Increased expression of anti-inflammatory cytokines in xenografts exposed to either NIC or NIC+HFD. (a) Western blot analysis of cytokines IL13, IL12, IL6, and MCSF in HCC70 xenografts obtained from various treatment groups. β-Actin was used as control. (b) Quantitative gene expression analysis of IL13 and IL6 in HCC70 xenografts from various treatment groups, using human- (right panel) and mouse- (left panel) specific primer sets. (c) Western blot analysis of p38MAPK and pERK1/2 in xenografts obtained from various treatment groups. (d) Immunohistochemical (IHC) analysis of IL13 expression in xenografts obtained from various treatment groups. andcompared to saline or ^#p ≤0.05 and ^##p ≤0.01 compared to the NIC group alone ().

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