Nicotine Synergizes with High-Fat Diet to Induce an Anti-Inflammatory Microenvironment to Promote Breast Tumor Growth

Figure 6

Effect of MEC and pp38MAPK inhibitor SB203580 on tumor growth in NIC and HFD-treated nude mice: HCC70 breast cancer cells (2×10⁶ cell/mice) were implanted in nude mice prefed on HFD (2 weeks) and exposed to NIC (intraperitoneal injection twice/day for 2 weeks, 0.75 mg/kg/mice/injection). Xenografts were grown in mice continued on either HFD ± NIC treatment (0.75 mg/kg/mice/injection twice a day) along with treatment with MEC (0.8 mg/kg/mice/injection, twice a day) with or without SB203580 (0.2 μmols in 100 μl/mice once/day/every day) till the tumors were excised. (a) Representative picture of xenografts after various treatments is shown. (b) Analysis of tumor volume following various treatments over a period of 8 weeks. (c) Analysis of tumor weight following various treatments over a period of 8 weeks. (d) Immunoblot analysis of ALDH1, SOX2, vimentin, and UPA in pooled tumors from various treatment groups (). and .

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