Baicalein Restores the Balance of Th17/Treg Cells via Aryl Hydrocarbon Receptor to Attenuate Colitis

Figure 5

Effect of baicalein on the differentiation of Th17 and Treg cells in vitro. Naïve CD4⁺ T cells were treated with anti-CD3/CD28 (2 μg/mL) and baicalein (5 and 10 μM), TCDD (5 nM), and CH223191 (10 μM) under Treg or Th17 cell differentiation condition for 24 h; percentages of CD4⁺ IL-17⁺ cells CD4⁺ CD25⁺ Foxp3⁺ cells were detected by flow cytometry. (a) Naïve CD4⁺ T cells were separated and identified by flow cytometry. (b) The viability and proliferation of naïve CD4⁺ T cells were detected by using MTT assays. (c, d) The percentages of CD4⁺ IL-17⁺ cells were detected by using flow cytometry. (e, f) The percentages of CD4⁺ CD25⁺ Foxp3⁺ cells were detected by flow cytometry. (g, h) The levels of IL-17 and IL-10 in the supernatant were detected by using ELISA. Experiments were repeated three times, and results were expressed as and vs. control model. CH: CH223191; CH+B: CH223191+baicalein.

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