Biological Characterization of Commercial Recombinantly Expressed Immunomodulating Proteins Contaminated with Bacterial Products in the Year 2020: The SAA3 Case

Figure 3

Impure mu rSAA3 induces chemokines in murine RAW264.7 macrophages, L929 fibroblasts and peritoneal cells. Murine RAW264.7 macrophages (a), murine L929 fibroblasts (b) and murine peritoneal cells derived from healthy mice (c) were stimulated with different concentrations of PGN (1 or 10 ng/ml; a and b), LPS (500 or 5000 ng/ml (a and b) or 50-5000 ng/ml (c)), hu rSAA1 (10-1000 ng/ml), mu rSAA3 (10-1000 ng/ml) or were left untreated (Co). After 24 h, supernatants were collected and the production of CCL2 (a and b), CXCL1 (b), CXCL2 (a and c) and CXCL6 (c) was determined via specific ELISAs. Data represent the mean production of derived from 4 to 5 (a and b) or 6 (c) independent experiments. Statistically significant differences from untreated control cells, determined by the Mann-Whitney test, are indicated by asterisks (; ).

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