Biological Characterization of Commercial Recombinantly Expressed Immunomodulating Proteins Contaminated with Bacterial Products in the Year 2020: The SAA3 Case

Figure 6

Low concentrations of LPS induce CXCL8 in CD14⁺ monocytes. Human CD14⁺ monocytes derived from buffy coats from healthy donors were stimulated with LPS (0.7-7000 pg/ml) or were left untreated (Co). After 24 h, supernatants were collected and levels of CXCL8 were determined via a specific ELISA. Data represent the mean production of derived from 3 to 11 independent experiments. Statistically significant differences from untreated control cells, determined by the Mann-Whitney test, are indicated by asterisks (; ; ).