Biological Characterization of Commercial Recombinantly Expressed Immunomodulating Proteins Contaminated with Bacterial Products in the Year 2020: The SAA3 Case

Figure 7

In contrast to impure mu rSAA3, RP-HPLC-purified mu rSAA3 (mu rSAA3_pur) does not induce chemokines in leukocytes. Human CD14⁺ monocytes derived from buffy coats from healthy individuals (a) or murine peritoneal cells derived from healthy mice (b) were stimulated with LPS (500 ng/ml), mu rSAA3 (10 or 100 ng/ml), mu rSAA3_pur (10 or 100 ng/ml) or were left untreated (Co). After 24 h, supernatants were collected and levels of CXCL8 (a) or CXCL2 (b) were determined via specific ELISAs. Data represent the mean production of derived from 4 (a) or 3 (b) independent experiments. Statistically significant differences from untreated control cells, determined by the Mann-Whitney test, are indicated by asterisks ().