Inhibition of miR-17~92 Cluster Ameliorates High Glucose-Induced Podocyte Damage

Figure 4

ABCA1 is a potential effector of the miR-17~92 cluster. (a) ABCA1 mRNA contains a site complementary to miR-17-5p and miR-20a-5p in the 3UTR region. (b, c) Luciferase activities of WT-ABCA1-3UTR and MUT-ABCA1-3UTR luciferase reporters after being transfected with miR-17-5p mimics (b) or miR-20a-5p mimics (c) were examined through the Dual-Luciferase Reporter Assay Kit. , vs, the NC-mimic transfection group. (d, e) The correlation among miR-17-5p, miR-20a-5p, and ABCA1 expression levels in the renal tissue samples (). (f, g) The expression of ABCA1 mRNA and protein was examined through RT-qPCR and Western blotting. In the Anta-miR group, cells were transfected with antagomirs of miR-17-5p and miR-20a-5p. , vs. the control group or Anta-NC group; ^#, vs the HG+Anta-NC group.

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