Carbon Monoxide-Releasing Molecule-3 Suppresses Tumor Necrosis Factor-α- and Interleukin-1β-Induced Expression of Junctional Molecules on Human Gingival Fibroblasts via the Heme Oxygenase-1 Pathway

Figure 1

Effect of CORM-3 on the viability of human gingival fibroblasts (HGFs). HGFs were stimulated with 0, 100, 200, 400, and 800 μM CORM-3 and 10 ng/ml TNF-α and 2 ng/m IL-1β for 24 h. Then, cell viability was assessed by Cell Counting Kit-8 (CCK-8) assays. The values were obtained from at least three independent experiments performed in triplicate, where and versus normal and noninduced HGFs and ^{^^^} compared between two groups, according to ANOVA followed by Tukey’s multiple comparison test. C: TNF-α- and IL-1β-induced cells; N: normal and noninduced HGFs. CORM-3: carbon monoxide-releasing molecule-3; OD: optical density; TNF-α: tumor necrosis factor-α; IL-1β: interleukin-1β.