Carbon Monoxide-Releasing Molecule-3 Suppresses Tumor Necrosis Factor-α- and Interleukin-1β-Induced Expression of Junctional Molecules on Human Gingival Fibroblasts via the Heme Oxygenase-1 Pathway

Figure 3

Location and expression changes of β-catenin, VE-cadherin, and ZO-1 in HGFs treated with CORM-3 in response to TNF-α and IL-1β stimulation. HGFs were stimulated with 10 ng/ml TNF-α and 2 ng/ml IL-1β with increasing concentrations of CORM-3 for 24 h, followed by confocal immunofluorescence assessment (original magnification ×400). DAPI (blue) was used for nuclear staining. TNF-α and IL-1β induction resulted in a decrease in β-catenin, VE-cadherin, and ZO-1 expressions in HGFs. The pictures are representative of three independent experiments. . C: TNF-α- and IL-1β-induced cells; N: normal and noninduced HGFs; ZO-1: zona occludens-1; CORM-3: carbon monoxide-releasing molecule-3; TNF-α: tumor necrosis factor-α; IL-1β: interleukin-1β; DAPI, 4,6-diamidino-2-phenylindole.