Carbon Monoxide-Releasing Molecule-3 Suppresses Tumor Necrosis Factor-α- and Interleukin-1β-Induced Expression of Junctional Molecules on Human Gingival Fibroblasts via the Heme Oxygenase-1 Pathway

Figure 4

Effects of CORM-3 on the expression of junctional complex molecules in HGFs following TNF-α and IL-1β induction. (a) Effects of CORM-3 on β-catenin, VE-cadherin, and ZO-1 mRNA expression in HGFs. (b) Protein expression of junctional complex molecules in HGFs following TNF-α and IL-1β induction. HGFs were stimulated with TNF-α (10 ng/ml) and IL-1β (2 ng/ml) and treated with increasing CORM-3 concentrations for 24 h. Total RNA or protein was extracted and analyzed using RT-qPCR or western blot analysis. The values were obtained from at least three independent experiments performed in triplicate, where , , and versus TNF-α- and IL-1β-induced cells and ^{^}, ^{^^}, and ^{^^^} compared between two groups, according to the ANOVA followed by Tukey’s multiple comparison test. C: TNF-α- and IL-1β-induced cells; N: normal and noninduced HGF; HGFs: human gingival fibroblasts; CORM-3: carbon monoxide-releasing molecule-3; TNF-α: tumor necrosis factor-α; IL-1β: interleukin-1β.

(a)

(b)